Role of antioxidants and amino acids on somatic embryogenesis of Pinus patula

被引：0
|
作者：
Ravindra B. Malabadi
Johannes Van Staden
机构：
[1] University of KwaZulu-Nata Pietermaritzburg,Research Centre for Plant Growth and Development, School of Botany and Zoology
来源：
In Vitro Cellular & Developmental Biology - Plant | 2005年 / 41卷
关键词：
antioxidant; ascorbic acid; citric acid; somatic embryogenesis; tissue necrosis;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
This study investigated the role of antioxidants and amino acids on somatic embryogenesis using the vegetative shoot apices of three genotypes of mature Pinus patula trees. Apart from dithiothreitol at 0.1%, pretreatment of explants or incorporation of antioxidants in the basal nutrient medium had a negative effect on the initiation of embryogenic cultures, somatic embryo production, and plantlet recovery. Inclusion of an amino acid solution mixture, at a concentration of 10.0 mM, in the maturation medium tended to increase somatic embryo production.
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页码：181 / 186
页数：5
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