Quantification of Amino Acids in Plasma by High-Performance Liquid Chromatography–Tandem Mass Spectrometry (LC–MS/MS)

For the extraction and measurement of plasma amino acids using LC/ESI–MS/MS technology, a straightforward and dependable approach has been established. The approach employs a small amount of sample and fully quantifies it using a labeled internal standard of amino acids. Water, formic acid, and methanol were used as the mobile phase in a gradient method to obtain clear separation. On a Shimatzu mass spectrometer 8030, optimized multiple reaction monitoring (MRM) was utilized to find amino acids. All amino acids have coefficients of correlation that range from 0.91 to 0.99, and they are all linear over their respective reference ranges. Precision’s intra-day and inter-day coefficients of variation (CV %) ranged from 3.29 to 11.73% and 5.04 to 12.48%, respectively. Amino acid recovery ranged from 92.1 to 108.2%.