Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

被引:1
|
作者
Meiyao Wang
Martin Misakian
Hua-Jun He
Peter Bajcsy
Fatima Abbasi
Jeffrey M Davis
Kenneth D Cole
Illarion V Turko
Lili Wang
机构
[1] University of Maryland,Institute for Bioscience and Biotechnology Research
[2] National Institute of Standards and Technology (NIST),Biomolecular Measurement Division
[3] NIST,Quantum Measurements Division
[4] NIST,Biosystems and Biomaterials Division
[5] NIST,Software and Systems Division
[6] CBER FDA,Laboratory of Stem Cell Biology, Cellular and Tissue Therapy Branch, Division of Cell and Gene Therapies
[7] NIST,Materials Measurement Science Division
来源
Clinical Proteomics | 2014年 / 11卷
关键词
Cryopreserved peripheral blood mononuclear cells (PBMC); Lyophilized Cyto-Trol™; CD4 receptor density; Flow cytometry; Multiple reaction monitoring (MRM) mass spectrometry (MS); Scanning electron microscopy (SEM); Microvilli; Cell surface area;
D O I
暂无
中图分类号
学科分类号
摘要
引用
收藏
相关论文
共 50 条
  • [1] Quantifying CD4 receptor protein in two human CD4+lymphocyte preparations for quantitative flow cytometry
    Wang, Meiyao
    Misakian, Martin
    He, Hua-Jun
    Bajcsy, Peter
    Abbasi, Fatima
    Davis, Jeffrey M.
    Cole, Kenneth D.
    Turko, Illarion V.
    Wang, Lili
    CLINICAL PROTEOMICS, 2014, 11
  • [2] CD4 lymphocyte count: TRAx CD4 test kit versus conventional flow cytometry.
    Saah, AJ
    Spruill, C
    Hoover, DR
    Prevots, R
    Taylor, E
    Margolick, JB
    Vlahov, D
    CLINICAL INFECTIOUS DISEASES, 1996, 23 (04) : 137 - 137
  • [3] Comparison of absolute CD4(+) lymphocyte counts determined by enzyme immunoassay (TRAx CD4 test kit) and flow cytometry
    Moss, MW
    Carella, AV
    Provost, V
    Quinn, TC
    CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1996, 3 (04) : 371 - 373
  • [4] Evaluation of a quantitative determination of CD4 and CD8 molecules as an alternative to CD4+ and CD8+ T lymphocyte counts in Africans
    Diagbouga, S
    Durand, G
    Sanou, PT
    Dahourou, H
    Ledru, E
    TROPICAL MEDICINE & INTERNATIONAL HEALTH, 1999, 4 (02) : 79 - 84
  • [5] Comparison of Advanced HIV Disease identification using CD4 results from a semi-quantitative CD4 point of care test and CD4 flow cytometry in Nigeria
    Otubu, N.
    Abudiore, O.
    Akanmu, M. -M.
    Levy-Braide, B.
    Eigege, W.
    Sowale, O.
    Inyang, A.
    Rathakrishnan, D.
    Amamilo, I.
    Conroy, J.
    Lufadeju, F.
    Amole, C.
    Wiwa, O.
    Nwaokenneya, P.
    Adesigbin, C.
    Ikpeazu, A.
    Olorunfemi, G.
    Oladele, R.
    Agbaji, O.
    Akanmu, S.
    JOURNAL OF THE INTERNATIONAL AIDS SOCIETY, 2022, 25 : 72 - 73
  • [6] A modified flow cytometry method for objective estimation of human CD4+ regulatory T cells (CD4+ Tregs) in peripheral blood, via CD4/CD25/CD45RO/FoxP3 labeling
    Petsiou, Asimina
    Paschou, Stavroula A.
    Vartholomatos, Georgios
    Chatzigianni, Katerina
    Kolaitis, Nikolaos
    Giotaki, Eleni
    Bondinas, George P.
    Moustakas, Antonis K.
    Karamoutsios, Achilleas
    Zervou, Eleftheria
    Tigas, Stelios
    Tsatsoulis, Agathocles
    Papadopoulos, George K.
    CYTOMETRY PART B-CLINICAL CYTOMETRY, 2020, 98 (03) : 259 - 269
  • [7] Optimal gating strategies for determining bronchoalveolar lavage CD4/CD8 lymphocyte ratios by flow cytometry
    Barry, SM
    Janossy, G
    JOURNAL OF IMMUNOLOGICAL METHODS, 2004, 285 (01) : 15 - 23
  • [8] CD4 LYMPHOCYTE ENUMERATION - COMPARISON BETWEEN FLOW-CYTOMETRY AND ENZYME-IMMUNOASSAY
    FEI, PC
    SOLMONE, M
    VANACORE, P
    GIGLIO, A
    BONIFATI, C
    CARDUCCI, M
    MUSSI, A
    CHIAROTTI, F
    AMEGLIO, F
    CYTOMETRY, 1995, 22 (01): : 70 - 74
  • [9] Affordable pediatric CD4 counting by flow cytometry in Malawi
    MacLennan, Calman A.
    Dzumani, Felix
    Namarika, Alinane
    Moons, Peter
    Senga, Edward
    Molyneux, Makohn E.
    Drayson, Mark T.
    Bunn, James E. G.
    CYTOMETRY PART B-CLINICAL CYTOMETRY, 2008, 74B : S90 - S97
  • [10] Evidence for a unique CD4 protein on murine vaginal CD4+ T cells.
    Wormley, FL
    Scott, M
    Luo, W
    Fidel, PL
    FASEB JOURNAL, 1998, 12 (04): : A614 - A614