Isolation and inhibitory effect of anti-Vibrio substances from Pseudoalteromonas sp. A1-J11 isolated from the coastal sea water of Kagoshima Bay

被引:0
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作者
Carmelo S del Castillo
Md Iftekharul Wahid
Takeshi Yoshikawa
Taizo Sakaia
机构
[1] Kagoshima University,The United Graduate School of Agricultural Sciences
[2] Kagoshima University,Laboratory of Microbiology, Faculty of Fisheries
来源
Fisheries Science | 2008年 / 74卷
关键词
anti-; substance; high-pressure liquid chromatography; ultraviolet absorption spectrum;
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摘要
Among several marine antagonistic bacteria isolated from the sea water of Kagoshima Bay, Pseudoalteromonas sp. A1-J11 was found to produce anti-Vibrio substances. The anti-Vibrio substances were extracted from the culture supernatant of the strain with chloroform and isolated using reverse-phase (Cosmosil 75C18-OPN) column chromatography followed by high-pressure liquid chromatography (Mightysil RP-18 GP Aqua). Purified substances, designated as AVS-03a, c and d showed similar ultraviolet absorption spectra with λmax at 215, 235, 315 and 327 nm in methanol. AVS-03d, the major anti-Vibrio substance, was thermostable up to 100°C and pH stable over a pH range higher than 4.0, and also showed strong inhibitory activities, specifically against Vibrio harveyi strains.
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页码:174 / 179
页数:5
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