Impact of Agrobacterium-infiltration and transient overexpression of BroMYB28 on glucoraphanin biosynthesis in broccoli leaves

被引：0

作者：

Young-Cheon Kim

Ahra Cha

Muhammad Hussain

Kwanuk Lee

Sanghyeob Lee

机构：

[1] Sejong University,Department of Bioindustry and Bioresource Engineering

[2] Ludwig-Maximilians-University Munich,Department Biology

[3] Plant Engineering Research Institute,undefined

[4] Sejong University,undefined

来源：

Plant Biotechnology Reports | 2020年 / 14卷

关键词：

Agrobacterium; Broccoli; Glucosinolate; Glucoraphanin; BroMYB28;

D O I：

暂无

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学科分类号：

摘要：

To date studies revealed that MYB28 intensively involved biosynthesis of GSLs, there is no direct evidence of MYB28 in glucoraphanin (GR) biosynthesis of broccoli. Furthermore, Agrobacterium (Agro)-infiltration also induced GSLs in Arabidopsis. However, there is no information regarding Agro-dependent GR accumulation in broccoli. We revealed that over-expression of BroMYB28 could accumulate GR, and Agro-infiltration is also enough to induce the accumulation of GR in broccoli. From our results, it is suggested that they used different regulatory mechanisms; BroMYB28 over-expression induced both BroMYB28 and BroMYB29 (Bol008849-like) transcripts; Agro-infiltration induced only BroMYB29 (Bol008849-like) transcripts.

引用

页码：373 / 380

页数：7