An enzyme immunoassay system for aflatoxin B1

被引:0
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作者
A. A. Burkin
G. P. Kononenko
N. A. Soboleva
E. V. Zotova
机构
[1] Russian Academy of Agricultural Sciences,Institute of Veterinary Sanitation, Hygiene and Ecology
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关键词
Apply Biochemistry; Aflatoxin; Carbodiimide; Sterigmatocystin; Aspergillus Parasiticus;
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摘要
Indirect enzyme immunoassay based on immobilized conjugate of aflatoxin B1 carboxymethyloxime with bovine serum albumin and polyclonal rabbit antibodies allows determining aflatoxin B1 with a low relative cross-reactivity against aflatoxin B2, G1, G2, M1 B2a, and G2a and sterigmatocystin (15.5, 15.5, 1.7, 1.0, 0.03, 0.03 and 0.01%, respectively) with a sensitivity of 0.04 ng per well or 4.0 ng per ml organic solvent.
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页码:80 / 84
页数:4
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