Biochemical characterization of dextranase from Arthrobacter oxydans and its cloning and expression in Escherichia coli

被引：0
|
作者：
Jin Ha Lee
Seong Hee Nam
Hyen Joung Park
Young-Min Kim
Nahyun Kim
Ghahyun Kim
Eun-Seong Seo
Seong-Soo Kang
Doman Kim
机构：
[1] Chonnam National University,School of Biological Sciences and Technology and Research Institute for Catalysis
[2] Chonnam National University,Dental Science Research Institute, School of Dentistry, 2nd Stage of Brain Korea 21 for School of Dentistry
[3] Jeonnam Agricultural Research and Extension Services,Major in Biochemistry and Cell Biology
[4] Cosmax Ltd.,College of Veterinary Medicine
[5] Korean Minjok Leadership Academy,undefined
[6] University of California-San Diego,undefined
[7] Chonnam National University,undefined
来源：
Food Science and Biotechnology | 2010年 / 19卷
关键词：
dextranase; dextran; sugar; expression;
D O I：
暂无
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学科分类号：
摘要：
Appreciably elevated levels of dextranase from Arthrobacter oxydans (AODex) isolated from sugar-cane farm soil was resulted from the culture on the Luria-Bertani (LB) medium containing 1%(w/v) soluble starch, glycerol, or dextran. The responsible gene (aodex) was cloned, its nucleotide sequence was determined, and expression of the encoded protein was achieved in Escherichia coli. An open reading frame was composed of 1,863 bp putatively encoding a 68.3 kDa protein. Recombinant A. oxydans dextranase (rAODex) was purified about 16 fold by nickel-nitrilotriacetic acid affinity column chromatography; Km value for dextran T2000 was 0.85 mg/mL (w/v). AODex treatment of stale sugar cane juice resulted in a yield of square and light-colored sugar crystals.
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页码：757 / 762
页数：5
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