Evaluation of the effect of late blight-resistant potato plants on the structure of bacterial associations in soil

被引:0
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作者
E. V. Zadorina
E. S. Boulygina
T. V. Kolganova
B. B. Kuznetsov
K. G. Skryabin
机构
[1] Russian Academy of Sciences,Bioengineering Center
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Transgenic Plant; Apply Biochemistry; Clone Library; Methanosarcina; nifH Gene;
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摘要
We studied the compositions of microbial associations isolated from soils where nontransgenic and transgenic late blight-resistant lines of potato varieties Lugovskoi, Charodei, and Golubizna had been grown. The analysis was based on denaturing gradient gel electrophoresis of total amplificates of 16S rRNA gene fragments and analysis of clone libraries of nifH gene fragments. Neither method revealed significant differences in the structure of the microbial associations isolated from soils with control or transgenic plants. The minor differences detected in the microflora ranges were no greater than those in the rhizospheres of different nontransgenic potato varieties.
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页码:194 / 198
页数:4
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