human colorectal cancer;
in situ hybridization;
macrophages;
tumour progression;
wnt genes;
D O I:
暂无
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学科分类号:
摘要:
Defects in the APC-β-catenin pathway are common in colon cancer. We investigated whether aberrant regulation of upstream ligands stimulating this pathway occur in colon cancer. Using RNAase protection analysis, six out of eight wnt genes were expressed in 14 matched cases of normal, adenomatous and malignant colorectal tissues. Wnt 2 and wnt 5a were significantly up-regulated in the progression from normal through adenoma to carcinoma. Transcripts for wnts 4, 7b, 10b and 13, but not wnt 2 and wnt 5a were detected in several colorectal cell lines. In situ hybridization demonstrated that wnt 2 and wnt 5a transcripts were mainly in the lamina propria/stroma region with labelling predominantly in macrophages. Immunostaining with CD68 confirmed the wnt-expressing cells as macrophages. These results show a major difference in wnt expression in colon cancer compared to colon adenomas and suggest stromal wnt expression may play a role in tumour progression.
机构:
Peking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R ChinaPeking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R China
Li, Bing
Shi, Xiao-Yu
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机构:
Peking Union Med Coll, State Key Lab Mol Oncol, Canc Inst Hosp, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Beijing 100021, Peoples R ChinaPeking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R China
Shi, Xiao-Yu
Liao, Dai-Xiang
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机构:
Peking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R ChinaPeking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R China
Liao, Dai-Xiang
Cao, Bang-Rong
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机构:
Peking Union Med Coll, State Key Lab Mol Oncol, Canc Inst Hosp, Beijing 100021, Peoples R China
Chinese Acad Med Sci, Beijing 100021, Peoples R ChinaPeking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R China
Cao, Bang-Rong
Luo, Cheng-Hua
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机构:
Peking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R ChinaPeking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R China
Luo, Cheng-Hua
Cheng, Shu-Jun
论文数: 0引用数: 0
h-index: 0
机构:
Peking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R ChinaPeking Univ, Sch Clin Med 9, Dept Surg Oncol, Ward 3,Beijing Shijitan Hosp,Capital Med Univ,Sch, Beijing 100038, Peoples R China
Cheng, Shu-Jun
INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,
2015,
8
(04):
: 4883
-
4898