Interrogating ligand-receptor interactions using highly sensitive cellular biosensors

被引：4

作者：

Funk M.A. ^{[1
]}

Leitner J. ^{[1
]}

Gerner M.C. ^{[2
]}

Hammerler J.M. ^{[2
]}

Salzer B. ^{[3
,4
]}

Lehner M. ^{[3
,4
]}

Battin C. ^{[1
]}

Gumpelmair S. ^{[1
]}

Stiasny K. ^{[5
]}

Grabmeier-Pfistershammer K. ^{[6
]}

Steinberger P. ^{[1
]}

机构：

[1] Center for Pathophysiology, Infectiology and Immunology, Institute of Immunology, Division for Immune Receptors and T cell activation, Medical University of Vienna, Vienna

[2] Division of Biomedical Science, University of Applied Sciences FH Campus Wien, Vienna

[3] St. Anna Children’s Cancer Research Institute, Vienna

[4] Christian Doppler Laboratory for Next Generation CAR T Cells, Vienna

[5] Center for Virology, Medical University of Vienna, Vienna

[6] Department of Dermatology, Medical University of Vienna, Vienna

来源：

Nature Communications | / 14卷 / 1期

基金：

奥地利科学基金会; 美国国家卫生研究院;

关键词：

D O I：

10.1038/s41467-023-43589-1

中图分类号：

学科分类号：

摘要：

Interactions of membrane-resident proteins are important targets for therapeutic interventions but most methods to study them are either costly, laborious or fail to reflect the physiologic interaction of membrane resident proteins in trans. Here we describe highly sensitive cellular biosensors as a tool to study receptor-ligand pairs. They consist of fluorescent reporter cells that express chimeric receptors harboring ectodomains of cell surface molecules and intracellular signaling domains. We show that a broad range of molecules can be integrated into this platform and we demonstrate its applicability to highly relevant research areas, including the characterization of immune checkpoints and the probing of cells for the presence of receptors or ligands. The platform is suitable to evaluate the interactions of viral proteins with host receptors and to test for neutralization capability of drugs or biological samples. Our results indicate that cellular biosensors have broad utility as a tool to study protein-interactions. © 2023, The Author(s).

引用

共 50 条

[1] Ligand-receptor interactions
Bongrand, P
REPORTS ON PROGRESS IN PHYSICS, 1999, 62 (06) : 921 - 968
[2] THE BIOPHYSICS OF LIGAND-RECEPTOR INTERACTIONS
DELISI, C
QUARTERLY REVIEWS OF BIOPHYSICS, 1980, 13 (02) : 201 - 230
[3] Understanding ligand-receptor interactions
Kubinyi, H
CHIMIA, 2003, 57 (1-2) : 57 - 58
[4] Discovering ligand-receptor interactions
Sarah A Slavoff
Alan Saghatelian
Nature Biotechnology, 2012, 30 : 959 - 961
[5] LIGAND-RECEPTOR INTERACTIONS IN WATER
MCCAMMON, JA
LYBRAND, TP
MAZOR, MH
WONG, CF
BIOPHYSICAL JOURNAL, 1986, 49 (02) : A1 - A1
[6] Discovering ligand-receptor interactions
Slavoff, Sarah A.
Saghatelian, Alan
NATURE BIOTECHNOLOGY, 2012, 30 (10) : 959 - 961
[7] Fractal kinetics for ligand-receptor binding measured with biosensors
Bajzer, Z
Orth, JD
BIOPHYSICAL JOURNAL, 2002, 82 (01) : 481A - 481A
[8] LIGAND-RECEPTOR INTERACTIONS - FACTS AND FANTASIES
KLOTZ, IM
QUARTERLY REVIEWS OF BIOPHYSICS, 1985, 18 (03) : 227 - 259
[9] Parathyroid hormone, ligand-receptor interactions
Chorev, M
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2005, 230 : U2654 - U2654
[10] Ligand-receptor Interactions by NMR Spectroscopy
Tepes, P.
Novak, P.
KEMIJA U INDUSTRIJI-JOURNAL OF CHEMISTS AND CHEMICAL ENGINEERS, 2008, 57 (04): : 165 - 173

← 1 2 3 4 5 →