Celestine blue as a new indicator in electrochemical DNA biosensors

被引：0

作者：

Ezat Hamidi-Asl

Jahan Bakhsh Raoof

Abdolraoof Samadi Meibodi

Zahra Haghnavaz Bazgir

机构：

[1] Eletroanalytical Chemistry Research Laboratory,Department of Analytical Chemistry

[2] Faculty of Chemistry,Department of Analytical Chemistry

[3] University of Mazandaran,undefined

[4] Spectroscopic Research Laboratory,undefined

[5] Faculty of Chemistry,undefined

[6] University of Mazandaran,undefined

来源：

Science China Chemistry | 2016年 / 59卷

关键词：

celestine blue; differential pulse voltammetry; DNA biosensor; electroactive indicator;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Celestine blue (CB) was introduced as a new electroactive indicator in DNA biosensors. The interaction of CB with DNA was investigated by electrochemical and spectroscopic methods. The effect of buffer kind and pH on the electrochemical behavior of CB was studied. The peak currents of CB were linearly related to DNA concentration in the range of 5.0×10-9 to 1.0×10-7 mol/L. The detection limit of this approach was 4.76×10-10 mol/L. Based on spectrometry data a hypochromic effect was observed in UV-Vis spectra of CB with increasing DNA concentration. The results illustrate the possible interaction mode between CB and DNA is electrostatic binding.

引用

页码：128 / 134

页数：6

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