Examination of the structure of agaricinic acid using 1H and 13C NMR spectroscopy

Agaricinic acid was extracted from the carpophore of Fomitopsis officinalis (Vill. Bond. et Sing). Its structure was established by 1H and 13C NMR spectroscopy and comparison to a standard sample (Sigma—Aldrich). The extraction was carried out using ethanol. The obtained extract was evaporated, cooled (−5°C), and purified by ether. The structure of unbranched alkyl radicals was determined using their proton spectrum. The 13C NMR spectrum and two-dimensional {13C,1H} correlation diagram showed the presence of a hydroxyl (second quaternary C atom) and three types of carboxy groups (first, second, and third C atoms). The spectrum of the extracted sample is identical to that of the standard sample of agaricinic acid.