Agrobacterium-mediated transformation of cork oak (Quercus suber L.) somatic embryos

被引：0
|
作者：
N. Sánchez
J. A. Manzanera
B. Pintos
M. A. Bueno
机构：
[1] Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA),
[2] Universidad Politecnica de Madrid,undefined
[3] E.T.S.I. Montes,undefined
来源：
New Forests | 2005年 / 29卷
关键词：
Genetic transformation; Hygromycin resistance; Marker gene; Reporter gene;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A simple method is presented for the unreported genetic transformation of cork oak (Quercus suber L.). Pro-embryo masses (PEMs) were induced on immature zygotic embryos applied to medium supplemented with 2.3 μM 2,4-dichlorophenoxyacetic acid. The established PEMs were inoculated with Agrobacterium tumefaciens LBA4404/p35S GUS INT/pCAMBIA 1301 strain. Transformants were selected on hygromycin (HYG) 94 μM-supplemented medium. Viable embryos constituted 13% of those selected on HYG during 4 months. Expression of β-glucuronidase at 4 months following co-cultivation confirmed transformation in 5.8% embryos selected on HYG. This method forms a basis for genetic transformation of cork oak somatic embryos.
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页码：169 / 176
页数：7
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