Monitoring protein conformational changes and dynamics using stable-isotope labeling and mass spectrometry

被引：0
|
作者：
Alem W Kahsai
Sudarshan Rajagopal
Jinpeng Sun
Kunhong Xiao
机构：
[1] Duke University Medical Center,Department of Medicine
[2] Howard Hughes Medical Institute,Department of Biochemistry and Molecular Biology
[3] Duke University Medical Center,undefined
[4] Shandong University,undefined
[5] School of Medicine,undefined
来源：
Nature Protocols | 2014年 / 9卷
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学科分类号：
摘要：
CDSiL-MS involves labeling cysteine and lysine side chains in proteins with N-ethylmaleimide and succinic anhydride, respectively. Information about the conformational state of the protein is inferred from the labeling kinetics as determined by mass spectrometry.
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页码：1301 / 1319
页数：18
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