2D [1H,13C] NMR study of carbon fluxes during glucose utilization by Escherichia coli MG1655

被引：0
|
作者：
A. D. Kivero
E. V. Bocharov
V. G. Doroshenko
A. G. Sobol
M. A. Dubinnyi
A. S. Arseniev
机构：
[1] Ajinomoto-Genetika Research Institute,Shemyakin
[2] Russian Academy of Sciences,Ovchinnikov Institute of Bioorganic Chemistry
来源：
Applied Biochemistry and Microbiology | 2008年 / 44卷
关键词：
Apply Biochemistry; Pentose Phosphate Pathway; Glucose Consumption; Strain MG1655; MG1655 MG1655;
D O I：
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学科分类号：
摘要：
Carbon fluxes through main pathways of glucose utilization in Escherichia coli cells-glycolysis, pentose phosphate pathway (PPP), and Enther-Doudoroff pathway (EDP)—were studied. Their ratios were analyzed in E. coli strains MG1655, MG1655Δ(edd-eda), MG1655Δ(zwf, edd-eda), and MG1655Δ(pgi, edd-eda). It was shown that the carbon flux through glycolysis was the main route of glucose utilization, averaging ca. 80%. Inactivation of EDP did not affect growth parameters. Nevertheless, it altered carbon fluxes through the tricarboxylic acid cycles and energy metabolism in the cell. Inactivation of PPP decreased growth rate to a lesser degree than glycolysis inactivation.
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页码：151 / 157
页数：6
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