Activity of DNA polymerase-λ in dry and germinating seeds of Zea mays L

被引：1
|
作者：
Bakshi S. [1 ]
Sihi S. [2 ]
Sengupta D.N. [2 ]
机构：
[1] Department of Botany, Vidyasagar College for Women, 39 Sankar Ghosh Lane, Kolkata, 700 006, West Bengal
[2] Division of Plant Biology, Bose Institute, 93/1 APC Road, Kolkata, 700 009, West Bengal
来源：
Indian Journal of Plant Physiology | 2015年 / 20卷 / 4期
关键词：
DNA polymerase; DNA repair; Seed germination; Zea mays;
D O I：
10.1007/s40502-015-0185-0
中图分类号：
学科分类号：
摘要：
Imbibition of water by dry seeds initiates active metabolic processes that lead to seed germination. Intact and damage free DNA is required in embryonic cells before the DNA replication in every cycle of cell division that occurs during seed germination. DNA polymerase-λ (pol-λ) is a dideoxynucleotidetriphosphate (ddNTP) sensitive single polypeptide enzyme involved in DNA repair and present in imbibed seeds of rice. Activity of pol-λ was studied in dry, imbibed and germinating seeds of Zea mays. DNA polymerase enzyme assay showed enhanced activity of the ddNTP sensitive enzyme after imbibition. Western blot analysis showed presence of the enzyme during seed germination. Prominent presence of the ddNTP sensitive DNA pol-λ was found after imbibition in Zea mays in activity gel analysis. Results indicate crucial role of the enzyme during seed germination and early seedling stage in maize plant. © 2015, Indian Society for Plant Physiology.
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收藏
页码：396 / 399
页数：3
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