DNA from Clarified, Large-Scale, Fed-Batch, Mammalian Cell Culture is of Predominantly Low Molecular Weight

The molecular weight of DNA from a large-scale, fed-batch, mammalian cell culture vessel has been evaluated as process material passes through the initial stages of a purification scheme for monoclonal antibodies. High molecular weight DNA was substantially cleared from the broth after passage through a disc stack centrifuge and the remaining low molecular weight DNA was largely unaffected by passage through a series of depth filters and a sterilising grade membrane. Removal of high molecular weight DNA was shown to be coupled with clarification of the process stream. The DNA from cell culture supernatant showed a pattern of internucleosomal cleavage of chromatin when fractionated by electrophoresis but the presence of both necrotic and apoptotic cells throughout the fermentation meant that the origin of the fragmented DNA could not be unequivocally determined.