Non-intercalative, Deoxyribose Binding of Boric Acid to Calf Thymus DNA

被引：0
|
作者：
Ayse Ozdemir
Refiye Tekiner Gursaclı
Turgay Tekinay
机构：
[1] Bilkent University,UNAM
[2] Gazi University,National Nanotechnology Research Center
[3] Gazi University,Life Sciences Research and Application Center
来源：
Biological Trace Element Research | 2014年 / 158卷
关键词：
Calf thymus DNA; Boric acid; Spectroscopy; Binding mode; FT-IR;
D O I：
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摘要：
The present study characterizes the effects of the boric acid binding on calf thymus DNA (ct-DNA) by spectroscopic and calorimetric methods. UV–Vis absorbance spectroscopy, circular dichroism (CD) spectroscopy, transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and Fourier transform infrared (FT-IR) spectroscopy were employed to characterize binding properties. Changes in the secondary structure of ct-DNA were determined by CD spectroscopy. Sizes and morphologies of boric acid–DNA complexes were determined by transmission electron microscopy (TEM). The kinetics of boric acid binding to calf thymus DNA (ct-DNA) was investigated by isothermal titration calorimetry (ITC). ITC results revealed that boric acid exhibits a moderate affinity to ct-DNA with a binding constant (Ka) of 9.54 × 104 M−1. FT-IR results revealed that boric acid binds to the deoxyribose sugar of DNA without disrupting the B-conformation at tested concentrations.
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页码：268 / 274
页数：6
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