Characterization and processing of prostate specific antigen (hK3) and human glandular kallikrein (hK2) secreted by LNCaP cells

被引：0
|
作者：
V Väisänen
J Lövgren
J Hellman
T Piironen
H Lilja
K Pettersson
机构：
[1] University of Turku,Department of Biotechnology
[2] Lund University,Department of Laboratory Medicine, Division for Clinical Chemistry
[3] University Hospital,undefined
来源：
Prostate Cancer and Prostatic Diseases | 1999年 / 2卷
关键词：
prostate specific antigen; kallikrein; proPSA; LNCaP; cancer;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Prostate specific antigen (PSA, hK3) in serum is predominantly complexed to α-1-antichymotrypsin (ACT), but a minor fraction remains in a free form despite the very large excess of serine protease inhibitors and α-2-macroglobulin. The fraction of free to total PSA is significantly lower in prostate cancer (CaP) compared to benign prostatic hyperplasia (BPH) which provides improved discrimination of these conditions. The molecular nature of free PSA in the circulation and the reason for its varying concentration in malignant and benign conditions is currently not known. The objective of the present investigation was to study the secretion of PSA and human glandular kallikrein 2 (hK2) by the LNCaP prostate cancer cell line, and to purify and characterize both proteins.
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页码：91 / 97
页数：6
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