Application of Laser Correlation Spectroscopy for Measuring Virus Size

被引:0
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作者
V. N. Nikiforov
S. E. Vinogradov
A. V. Ivanov
E. V. Efremova
L. B. Kalnina
A. B. Bychenko
Yu. Yu. Tentsov
A. A. Manykin
机构
[1] M. V. Lomonosov Moscow State University,Physical Faculty
[2] Russian Academy of Medical Sciences,N. N. Blokhin Russian Cancer Research Center
[3] Ministry of Health of the Russian Federation,D. I. Ivanovsky Institute of Virology, N. F. Gamaleya Federal Center of Epidemiology and Microbiology
[4] M. P. Chumakov Institute of Poliomyelitis and Viral Encephalitides,undefined
关键词
virus size; HIV-1; hepatitis A virus; laser correlation spectroscopy (dynamic light scattering); electron microscopy;
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摘要
Dynamic light scattering method or laser correlation spectroscopy was applied to evaluation of the size of viruses. We measured correlation functions of the light scattered by human immunodeficiency viruses (HIV) and hepatitis A viruses (HAV) and found that size of HIV-1 (subtype A and B) and HAV virions were 104 nm and 28 nm, respectively. Comparison of these findings with electron microscopy data for fixed samples of the same viruses showed good agreement of the results.
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页码:88 / 91
页数:3
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