Real-time PCR for differential determination of the tomato wilt fungus, Fusarium oxysporum f. sp. lycopersici, and its races

被引：0
|
作者：
Keigo Inami
Chizu Yoshioka
Yasushi Hirano
Masato Kawabe
Seiya Tsushima
Tohru Teraoka
Tsutomu Arie
机构：
[1] Tokyo University of Agriculture and Technology (TUAT),Laboratory of Plant Pathology
[2] Saitama Prefectural Agriculture and Forestry Research Center,undefined
[3] National Agricultural Research Center (NARC),undefined
[4] National Institute for Agro-Environmental Sciences (NIAS),undefined
来源：
Journal of General Plant Pathology | 2010年 / 76卷
关键词：
Avirulence gene; Detection; rDNA-intergenic spacer (IGS) region; Soil DNA;
D O I：
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中图分类号：
学科分类号：
摘要：
Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races.
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页码：116 / 121
页数：5
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