A Method for Determining Characteristic Parameters of DNA Melting in Nucleic Acid Analyzers

被引：0

作者：

V. E. Kurochkin

D. A. Belov

Yu. V. Belov

A. N. Zubik

机构：

[1] Russian Academy of Sciences,Institute for Analytical Instrumentation

来源：

Biomedical Engineering | 2022年 / 55卷

关键词：

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Polymerase chain reaction (PCR) and DNA melting methods are run on nucleic acid analyzers. We propose a method for recording melting signals using analyzers of the ANK series that decreases errors in determining the main parameter – the DNA melting temperature Tm. We propose a method for assessing the width of the melting interval ΔT as an additional specificity criterion for PCR products. Measurement errors have been evaluated: standard deviation (RMS) for Tm, ≤0.11°C; for ΔT, ≤0.06°C.

引用

页码：333 / 336

页数：3

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