Purification and preliminary characterization of a xylanase from Thermomyces lanuginosus strain SS-8

被引：0
|
作者：
Smriti Shrivastava
Pratyoosh Shukla
Kunal Mukhopadhyay
机构：
[1] Birla Institute of Technology (Deemed University),Enzyme Technology Laboratory, Department of Biotechnology
来源：
3 Biotech | 2011年 / 1卷
关键词：
Bioethanol; Thermophilic fungi; Xylanase; Xylose;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
Thermomyces lanuginosus SS-8 was isolated from soil samples that had been collected from near self-heating plant material and its extracellular cellulase-free xylanase purified approximately 160-fold using ion exchange chromatography and continuous elution electrophoresis. This xylanase was thermoactive (optimum temperature 60 °C) at pH 6.0 and had a molecular weight of 23.79 kDa as indicated by SDS-PAGE electrophoresis. The xylanase rapidly hydrolyzed xylan directly to xylose without the production of intermediary xylo-oligosaccharides within 15 min of incubation under optimum conditions. This trait of rapidly degrading xylan to xylose as a sole end-product could have biotechnological potential in degradation of agro-wastes for bioethanol manufacturing industry.
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页码：255 / 259
页数：4
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