Micropropagation of Clitoria ternatea Linn. (Fabaceae)— An important medicinal plant

被引:0
|
作者
G. R. Rout
机构
[1] Regional Plant Resource Centre,Plant Biotechnology Division, Plant Tissue Culture Laboratory
关键词
growth regulators; medicinal plant; shoot multiplication;
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学科分类号
摘要
An efficient protocol was established for in vitro shoot multiplication from nodal explants of Clitoria ternatea on semisolid Murashige and Skoog (MS) basal medium supplemented with 8.9μM 6-benzylaminopurine (BA). Inclusion of 1-naphthaleneacetic acid (NAA) in the culture medium along with BA promoted higher rates of shoot multiplication than BA alone. The rate of shoot multiplication was maximum (5.21) after 4 wk of culture on MS basal medium supplemented with 8.9μM BA and 1.34μM NAA. The elongated shoots rooted within 7–8d in half-strength MS basal salts supplemented with 1.34μM NAA and 2% (w/v) sucrose. About 85% of the rooted plantlets were acclimatized and transferred to the greenhouse.
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页码:516 / 519
页数:3
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