Improved production of recombinant rotavirus VP6 in sodium butyrate-supplemented suspension cultures of transgenic tomato (Lycopersicon esculentum Mill.) cells

Recombinant rotavirus VP6 production was 0.49 mg l−1 when 10 mM sodium butyrate was added to the suspension culture of transgenic tomato cells after 6 days' incubation. Recombinant VP6 production increased to 0.73 mg l−1, when the culture was supplemented with 10 mM sodium butyrate on day 6 and 9 after incubation. Total protein extracts of transgenic tomato cells from sodium butyrate-supplemented culture were examined by two-dimensional electrophoresis: 14 proteins were up-regulated and 7 down-regulated.