A simple method for long-term storage of Acanthamoeba species

被引：0
|
作者：
Diana Axelsson-Olsson
Jenny Olofsson
Patrik Ellström
Jonas Waldenström
Björn Olsen
机构：
[1] University of Kalmar,Section for Zoonotic Ecology and Epidemiology
[2] Uppsala University Hospital,Section of Infectious Diseases, Department of Medical Sciences
来源：
Parasitology Research | 2009年 / 104卷
关键词：
Confluent Layer; Axenic Culture; Storage Method; Acanthamoeba Castellanii; Refrigerator Temperature;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
We present a novel and simple technique for storing live Acanthamoeba for long periods of time. The amoebae are maintained at refrigerator temperatures in a peptone–yeast extract–glucose (PYG) medium normally used for cultivation. Using this method, we obtained survival rates of at least 4 years for Acanthamoeba polyphaga and 3 years for Acanthamoeba castellanii and Acanthamoeba rhysodes. Advantages of this storage method are: (1) it is quick and simple, (2) inexpensive, (3) does not require encystment before storage, (4) resuscitation of cysts can be achieved within a week of culture in PYG medium at 27°C, and does not require co-culture with bacteria or any special equipment.
引用
收藏
页码：935 / 937
页数：2
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