Annotation and characterization of Cd-responsive metal transporter genes in rapeseed (Brassica napus)

被引:0
|
作者
Xian Duo Zhang
Jin Guo Meng
Kai Xuan Zhao
Xi Chen
Zhi Min Yang
机构
[1] Nanjing Agricultural University,Department of Biochemistry and Molecular Biology, College of Life Sciences
来源
BioMetals | 2018年 / 31卷
关键词
Cadmium; Heavy metal transporters; Transcriptome;
D O I
暂无
中图分类号
学科分类号
摘要
In higher plants, heavy metal transporters are responsible for metal uptake, translocation and homeostasis. These metals include essential metals such as zinc (Zn) or manganese (Mn) and non-essential metals like cadmium (Cd) or lead (Pb). Although a few heavy metal transporters have been well identified in model plants (e.g. Arabidopsis and rice), little is known about their functionality in rapeseed (Brassica napus). B. napus is an important oil crop ranking the third largest sources of vegetable oil over the world. Importantly, B. napus has long been considered as a desirable candidate for phytoremediation owning to its massive dry weight productivity and moderate to high Cd accumulation. In this study, 270 metal transporter genes (MTGs) from B. napus genome were identified and annotated using bioinformatics and high-throughput sequencing. Most of the MTGs (74.8%, 202/270) were validated by RNA-sequencing (RNA-seq) the seedling libraries. Based on the sequence identity, nine superfamilies including YSL, OPT, NRAMP, COPT, ZIP, CDF/MTP, HMA, MRP and PDR have been classified. RNA-sequencing profiled 202 non-redundant MTGs from B. napus seedlings, of which, 108 MTGs were differentially expressed and 62 genes were significantly induced under Cd stress. These differentially expressed genes (DEGs) are dispersed in the rapeseed genome. Some of the genes were well confirmed by qRT-PCR. Analysis of the genomic distribution of MTGs on B. napus chromosomes revealed that their evolutional expansion was probably through localized allele duplications.
引用
收藏
页码:107 / 121
页数:14
相关论文
共 50 条
  • [1] Annotation and characterization of Cd-responsive metal transporter genes in rapeseed (Brassica napus)
    Zhang, Xian Duo
    Meng, Jin Guo
    Zhao, Kai Xuan
    Chen, Xi
    Yang, Zhi Min
    BIOMETALS, 2018, 31 (01) : 107 - 121
  • [2] Identification of Cd-responsive ATP binding cassette (ABC) transporter genes in rapeseed (Brassica napus)
    Yang, Zhimin
    Zhang, Xian Duo
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 256
  • [3] Identification of genomic ATP binding cassette (ABC) transporter genes and Cd-responsive ABCs in Brassica napus
    Zhang, Xian Duo
    Zhao, Kai Xuan
    Yang, Zhi Min
    GENE, 2018, 664 : 139 - 151
  • [4] Genome-wide identification of Cd-responsive NRAMP transporter genes and analyzing expression of NRAMP 1 mediated by miR167 in Brassica napus
    Meng, Jin Guo
    Zhang, Xian Duo
    Tan, Shang Kun
    Zhao, Kai Xuan
    Yang, Zhi Min
    BIOMETALS, 2017, 30 (06) : 917 - 931
  • [5] Genome-wide identification of Cd-responsive NRAMP transporter genes and analyzing expression of NRAMP 1 mediated by miR167 in Brassica napus
    Jin Guo Meng
    Xian Duo Zhang
    Shang Kun Tan
    Kai Xuan Zhao
    Zhi Min Yang
    BioMetals, 2017, 30 : 917 - 931
  • [6] Identification of Cd-responsive RNA helicase genes and expression of a putative BnRH 24 mediated by miR158 in canola (Brassica napus)
    Zhang, Xian Duo
    Sun, Jia Yun
    You, Yuan Yuan
    Song, Jian Bo
    Yang, Zhi Min
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2018, 157 : 159 - 168
  • [7] Genome-Wide Identification and Characterization of Ammonium Transporter (AMT) Genes in Rapeseed (Brassica napus L.)
    Dai, Jing
    Han, Peipei
    Walk, Thomas C.
    Yang, Ling
    Chen, Liyu
    Li, Yinshui
    Gu, Chiming
    Liao, Xing
    Qin, Lu
    GENES, 2023, 14 (03)
  • [8] FRACTIONATION AND CHARACTERIZATION OF POLYSACCHARIDES IN RAPESEED (BRASSICA-NAPUS) MEAL
    THEANDER, O
    AMAN, P
    SWEDISH JOURNAL OF AGRICULTURAL RESEARCH, 1977, 7 (02): : 69 - 77
  • [9] Characterization of allergens from rapeseed pollen and seed (Brassica napus)
    Godfrin, D.
    Senechal, H.
    Haddad, I.
    Sutra, J.
    Chardin, H.
    Desvaux, F.
    Vinh, J.
    Peltre, G.
    ALLERGY, 2007, 62 : 293 - 293
  • [10] The identification and characterization of heavy metal stress responsive genes in Brassica juncea
    Dalyan, E.
    Yuksel, B.
    Yuzbasioglu, E.
    Unal, M.
    FEBS JOURNAL, 2014, 281 : 691 - 691