Detection of bacteria by surface-enhanced Raman spectroscopy

被引：0
|
作者：
Atanu Sengupta
Mirna Mujacic
E. James Davis
机构：
[1] University of Washington,Department of Chemistry
[2] University of Washington,Department of Chemical Engineering
来源：
Analytical and Bioanalytical Chemistry | 2006年 / 386卷
关键词：
Bacteria detection; Bacterial pathogens ; Raman spectroscopy;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The detection and identification of dilute bacterial samples by surface-enhanced Raman spectroscopy has been explored by mixing aqueous suspensions of bacteria with a suspension of nanocolloidal silver particles. An estimate of the detection limit of E. coli was obtained by varying the concentration of bacteria. By correcting the Raman spectra for the broad librational OH band of water, reproducible spectra were obtained for E. coli concentrations as low as approximately 103 cfu/mL. To aid in the assignment of Raman bands, spectra for E. coli in D2O are also reported.
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页码：1379 / 1386
页数：7
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