Modern sequencing technologies and their applications

被引：0
|
作者：
Dobner J. [1 ]
Rossi A. [1 ]
机构：
[1] Genome Engineering and Model Development, IUF - Leibniz-Institut für umweltmedizinische Forschung, Auf’m Hennekamp 50, Düsseldorf
来源：
BIOspektrum | 2023年 / 29卷 / 5期
关键词：
D O I：
10.1007/s12268-023-1989-5
中图分类号：
学科分类号：
摘要：
CRISPR-mediated genome editing enables to study disease-relevant mutations. Next Generation Sequencing offers high throughput and accuracy but requires substantial investment. Nanopore sequencing on the other hand provides cheap entry. Here, we describe our work using sequencing techniques to identify edited cells, analyze mitochondrial DNA, and gene expression. Finally, we describe how our software tools CRISPRnano and Duesselpore aid to democratize the use of modern sequencing technologies. © 2023, Die Autoren.
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收藏
页码：479 / 482
页数：3
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