Development and application of a duplex PCR for the detection of Aspergillus carbonarius occurring in grapes

被引：0
|
作者：
Raffaele Lopardo
Mauro Musto
Francesco Cellini
Pasquale Domenico Grieco
机构：
[1] Metapontum Agrobios,
来源：
Annals of Microbiology | 2011年 / 61卷
关键词：
Duplex PCR; DNA extraction method; Detection tool;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
In this study, the development of a duplex PCR for the specific detection of Aspergillus carbonarius—a potent ochratoxin A (OTA) producer—is described. This approach resulted in a very accurate and sensitive method for the detection of low amounts of the mycotoxigenic mould occurring in grapes. In addition, an optimised protocol for the isolation of genomic DNA from a polyphenol-rich fruit like grape was developed.
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页码：5 / 9
页数：4
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