Comparative mapping in watermelon [Citrullus lanatus (Thunb.) Matsum. et Nakai]

被引:0
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作者
Katherine Sandlin
Jason Prothro
Adam Heesacker
Nelly Khalilian
Rebecca Okashah
Wenwen Xiang
Eleni Bachlava
David G. Caldwell
Chris A. Taylor
Danelle K. Seymour
Victoria White
Eva Chan
Greg Tolla
Cathy White
Dolores Safran
Elaine Graham
Steven Knapp
Cecilia McGregor
机构
[1] University of Georgia,Institute of Plant Breeding, Genetics and Genomics
[2] Monsanto,Department of Molecular Biology
[3] Monsanto,Crop and Soil Science Department
[4] Max Planck Institute for Developmental Biology,Department of Horticulture and Institute of Plant Breeding, Genetics and Genomics
[5] Oregon State University,undefined
[6] University of Georgia,undefined
来源
关键词
Quantitative Trait Locus; Recombinant Inbred Line; Segregation Distortion; Single Nucleotide Polymorphism Marker; Fruit Size;
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摘要
The first single-nucleotide polymorphism (SNP) maps for watermelon [Citrullus lanatus (Thunb.) Matsum. et Nakai] were constructed and compared. Three populations were developed from crosses between two elite cultivars, Klondike Black Seeded × New Hampshire Midget (KBS × NHM), an elite cultivar and wild egusi accession, Strain II × PI 560023 (SII × Egusi) and an elite cultivar and a wild citron accession, ZWRM50 × PI 244019 (ZWRM × Citroides). The SII × Egusi and ZWRM × Citroides F2 populations consisted of 187 and 182 individuals respectively while the KBS × NHM recombinant inbred line (RIL) population consisted of 164 lines. The length of the genetic maps were 1,438, 1,514 and 1,144 cM with average marker distances of 3.8, 4.2, and 3.4 cM for the KBS × NHM, SII × Egusi and ZWRM × Citroides populations, respectively. Shared markers were used to align the three maps so that the linkage groups (LGs) represented the 11 chromosomes of the species. Marker segregation distortion were observed in all three populations, but was highest (12.7  %) in the ZWRM × Citroides population, where Citroides alleles were favored. The three maps were used to construct a consensus map containing 378 SNP markers with an average distance of 5.1 cM between markers. Phenotypic data was collected for fruit weight (FWT), fruit length (FL), fruit width (FWD), fruit shape index (FSI), rind thickness (RTH) and Brix (BRX) and analyzed for quantitative trait loci (QTL) associated with these traits. A total of 40 QTL were identified in the three populations, including major QTL for fruit size and shape that were stable across genetic backgrounds and environments. The present study reports the first SNP maps for Citrullus and the first map constructed using two elite parents. We also report the first stable QTL associated with fruit size and shape in Citrullus lanatus. These maps, QTL and SNPs should be useful for the watermelon community and represent a significant step towards the potential use of molecular tools in watermelon breeding.
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页码:1603 / 1618
页数:15
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