Canine Parvovirus Type 2a (CPV-2a)-Induced Apoptosis in MDCK Involves Both Extrinsic and Intrinsic Pathways

被引：0
|
作者：
Juwar Doley
Lakshya Veer Singh
G. Ravi Kumar
Aditya Prasad Sahoo
Lovleen Saxena
Uttara Chaturvedi
Shikha Saxena
Rajiv Kumar
Prafull Kumar Singh
R. S. Rajmani
Lakshman Santra
S. K. Palia
S. Tiwari
D. R. Harish
Arvind Kumar
G. S. Desai
Smita Gupta
Shishir K. Gupta
A. K. Tiwari
机构：
[1] Indian Veterinary Research Institute,Molecular Biology Lab, Division of Veterinary Biotechnology
来源：
Applied Biochemistry and Biotechnology | 2014年 / 172卷
关键词：
Apoptosis; Caspase; CPV-2; MDCK cells; Apoptotic pathways;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
The canine parvovirus type 2 (CPV-2) causes an acute disease in dogs. It has been found to induce cell cycle arrest and DNA damage leading to cellular lysis. In this paper, we evaluated the apoptotic potential of the “new CPV-2a” in MDCK cells and elucidated the mechanism of the induction of apoptosis. The exposure of MDCK cells to the virus was found to trigger apoptotic response. Apoptosis was confirmed by phosphatidylserine translocation, DNA fragmentation assays, and cell cycle analysis. Activation of caspases-3, -8, -9, and -12 and decrease in mitochondrial potential in CPV-2a-infected MDCK cells suggested that the CPV-2a-induced apoptosis is caspase dependent involving extrinsic, intrinsic, and endoplasmic reticulum pathways. Increase in p53 and Bax/Bcl2 ratio was also observed in CPV-2a-infected cells.
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页码：497 / 508
页数：11
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