Proteomic analysis of differential expression of lung proteins in response to highly pathogenic avian influenza virus infection in chickens

被引:0
|
作者
Periyasamy Vijayakumar
Ashwin Ashok Raut
Santhalembi Chingtham
Harshad V. Murugkar
Diwakar D. Kulkarni
Richa Sood
Vijendra Pal Singh
Anamika Mishra
机构
[1] ICAR-National Institute of High-Security Animal Diseases,Pathogenomics Laboratory
[2] OIE Reference lab for Avian Influenza,undefined
[3] ICAR -National Institute of High-Security Animal Diseases,undefined
[4] OIE Reference lab for Avian Influenza,undefined
[5] Veterinary College and Research Institute,undefined
[6] Tamil Nadu Veterinary and Animal Sciences University,undefined
来源
Archives of Virology | 2022年 / 167卷
关键词
D O I
暂无
中图分类号
学科分类号
摘要
Elucidation of the molecular pathogenesis underlying virus-host interactions is important for the development of new diagnostic and therapeutic strategies against highly pathogenic avian influenza (HPAI) virus infection in chickens. However, the pathogenesis of HPAI virus in chickens is not completely understood. To identify the intracellular signaling pathways and critical host proteins associated with influenza pathogenesis, we analyzed the lung proteome of a chicken infected with HPAI H5N1 virus (A/duck/India/02CA10/2011/Agartala). Mass spectrometry data sets were searched against the chicken UniProt reference database. At the local false discovery rate level of 5%, a total of 3313 proteins with the presence of at least one unique peptide were identified in the chicken lung proteome datasets. Differential expression analysis of these proteins showed that 247 and 1754 proteins were downregulated at 12 h and 48 h postinfection, respectively. We observed expression of proteins of the predominant signaling pathways, including Toll-like receptors (TLRs), retinoic acid-inducible gene I-like receptors (RLRs), NOD-like receptors (NLRs), and JAK-STAT signaling. Activation of these pathways is associated with the cytokine storm effect and thus may be the cause of the severity of HPAI H5N1 infection in chickens. We also observed the expression of myeloid differentiation primary response protein (MyD88), inhibitor of nuclear factor kappa B kinase subunit beta (IKBKB), interleukin 1 receptor associated kinase 4 (IRAK4), RELA proto-oncogene NF-κB subunit (RELA), and mitochondrial antiviral signaling protein (MAVS), which are involved in critical signaling pathways, as well as other, less-commonly identified proteins such as hepatocyte nuclear factor 4 alpha (HNF4A), ELAV-like RNA binding protein 1 (ELAVL1), fibronectin 1 (FN1), COP9 signalosome subunit 5 (COPS5), cullin 1 (CUL1), breast cancer type 1 susceptibility protein (BRCA1), and the FYN proto-oncogene Src family tyrosine kinase (FYN) as main hub proteins that might play important roles in influenza pathogenesis in chickens. In summary, we identified the signaling pathways and the proteomic determinants associated with disease pathogenesis in chickens infected with HPAI H5N1 virus.
引用
收藏
页码:141 / 152
页数:11
相关论文
共 50 条
  • [1] Proteomic analysis of differential expression of lung proteins in response to highly pathogenic avian influenza virus infection in chickens
    Vijayakumar, Periyasamy
    Raut, Ashwin Ashok
    Chingtham, Santhalembi
    Murugkar, Harshad, V
    Kulkarni, Diwakar D.
    Sood, Richa
    Singh, Vijendra Pal
    Mishra, Anamika
    [J]. ARCHIVES OF VIROLOGY, 2022, 167 (01) : 141 - 152
  • [2] Subclinical Highly Pathogenic Avian Influenza Virus Infection in Vaccinated Chickens, China
    Ma, Qing-Xia
    Jiang, Wen-Ming
    Liu, Shuo
    Wang, Su-Chun
    Zhuang, Qing-Ye
    Hou, Guang-Yu
    Liu, Xiang-Ming
    Sui, Zheng-Hong
    Chen, Ji-Ming
    [J]. EMERGING INFECTIOUS DISEASES, 2014, 20 (12) : 2152 - 2154
  • [3] Proteomics Analysis of Duck Lung Tissues in Response to Highly Pathogenic Avian Influenza Virus
    Vijayakumar, Periyasamy
    Mishra, Anamika
    Deka, Ram Pratim
    Pinto, Sneha M.
    Subbannayya, Yashwanth
    Sood, Richa
    Prasad, Thottethodi Subrahmanya Keshava
    Raut, Ashwin Ashok
    [J]. MICROORGANISMS, 2024, 12 (07)
  • [4] Effect of Infection with a Mesogenic Strain of Newcastle Disease Virus on Infection with Highly Pathogenic Avian Influenza Virus in Chickens
    Costa-Hurtado, Mar
    Afonso, Claudio L.
    Miller, Patti J.
    Shepherd, Eric
    DeJesus, Eric
    Smith, Diane
    Pantin-JackwoodA, Mary J.
    [J]. AVIAN DISEASES, 2016, 60 (01) : 269 - 278
  • [5] LESIONS IN BROILER AND LAYER CHICKENS IN AN OUTBREAK OF HIGHLY PATHOGENIC AVIAN INFLUENZA-VIRUS INFECTION
    ACLAND, HM
    BACHIN, LAS
    ECKROADE, RJ
    [J]. VETERINARY PATHOLOGY, 1984, 21 (06) : 564 - 569
  • [6] Primary Chicken and Duck Endothelial Cells Display a Differential Response to Infection with Highly Pathogenic Avian Influenza Virus
    Tong, Zhen Wei Marcus
    Karawita, Anjana C.
    Kern, Colin
    Zhou, Huaijun
    Sinclair, Jane E.
    Yan, Limin
    Chew, Keng Yih
    Lowther, Sue
    Trinidad, Lee
    Challagulla, Arjun
    Schat, Karel A.
    Baker, Michelle L.
    Short, Kirsty R.
    [J]. GENES, 2021, 12 (06)
  • [7] Analysis of the crow lung transcriptome in response to infection with highly pathogenic H5N1 avian influenza virus
    Vijayakumar, Periyasamy
    Mishra, Anamika
    Ranaware, Pradip B.
    Kolte, Atul P.
    Kulkarni, Diwakar D.
    Burt, David W.
    Raut, Ashwin Ashok
    [J]. GENE, 2015, 559 (01) : 77 - 85
  • [8] Protection of Chickens against Highly Pathogenic Avian Influenza
    Mori, Hisashi
    [J]. SEN-I GAKKAISHI, 2011, 67 (07) : P177 - P180
  • [9] Highly Pathogenic Avian Influenza Virus Infection in Feral Raccoons, Japan
    Horimoto, Taisuke
    Maeda, Ken
    Murakami, Shin
    Kiso, Maki
    Iwatsuki-Horimoto, Kiyoko
    Sashika, Mariko
    Ito, Toshihiro
    Suzuki, Kazuo
    Yokoyama, Mayumi
    Kawaoka, Yoshihiro
    [J]. EMERGING INFECTIOUS DISEASES, 2011, 17 (04) : 714 - 717
  • [10] Candidate Genes Associated with Survival Following Highly Pathogenic Avian Influenza Infection in Chickens
    Drobik-Czwarno, Wioleta
    Wolc, Anna
    Petal, Callie R.
    Miedzinska, Katarzyna
    Dekkers, Jack
    Fulton, Janet E.
    Smith, Jacqueline
    [J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2024, 25 (18)