Introduction and Expression of Cowpea Trypsin Inhibitor (CpTI) Gene in Transgenic Tobacco

被引：0

作者：

Durba Ghoshal

S. K. Sen

Arun Goyal

机构：

[1] Bose Institute,Plant Molecular and Cellular Genetics

[2] University of Minnesota Duluth,Laboratory of Plant Physiology, Biochemistry and Biotechnology, Department of Biology, and Department of Biochemistry and Molecular Biology

[3] Indian Institute of Technology,undefined

来源：

Journal of Plant Biochemistry and Biotechnology | 2001年 / 10卷

关键词：

cowpea; CpTI; insect resistance; trypsin inhibitor; transgenic plants;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A trypsin/chymotrypsin inhibitor (CpTI) was purified from mature cowpea seeds. A full length cDNA clone encoding the trypsin/chymotrypsin inhibitor protein was isolated from a cDNA library that was constructed using poly(A+) RNA from developing seeds. Tobacco protoplasts were transformed with a construct composed of CaMV 35S promoter, NPTII gene, CpTI gene, and NOS terminator, using polyethylene glycol (PEG)-mediated direct gene transfer. The CpTI gene has been integrated into the plant genome, and the expressed CpTI protein from transgenic plants was catalytically active.

引用

页码：19 / 24

页数：5

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