Fluorescence resonance energy transfer in ferritin labeled with multiple fluorescent dyes

被引：0

作者：

Belén Fernández

Natividad Gálvez

Purificación Sánchez

Rafael Cuesta

Ruperto Bermejo

José M. Domínguez-Vera

机构：

[1] Universidad de Granada,Departamento de Química Inorgánica, Facutad de Ciencias

[2] EPS Linares,Departamento de Química Inorgánica y Orgánica

[3] EPS Linares,Departamento de Química Física y Analítica

来源：

JBIC Journal of Biological Inorganic Chemistry | 2008年 / 13卷

关键词：

Ferritin; Fluorescence resonance energy transfer; Fluorescence; Alexa Fluor;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

We simultaneously labeled ferritin with two Alexa Fluor fluorophores (AF350 and AF430). When both fluorophores label the same ferritin subunit, fluorescence resonance energy transfer (FRET) takes place from the excited AF350 to the acceptor AF430. By varying the number and the ratio of labeling fluorophores, we can modulate FRET such that the ferritin particles can exhibit multiple colors under UV illumination. Labeling of the ferritin shell does not affect the properties of the metallic core.

引用

页码：349 / 355

页数：6

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