Efficient ΦC31 integrase–mediated site-specific germline transformation of Anopheles gambiae

被引：0
|
作者：
Emilie Pondeville
Nicolas Puchot
Janet M Meredith
Amy Lynd
Kenneth D Vernick
Gareth J Lycett
Paul Eggleston
Catherine Bourgouin
机构：
[1] Institut Pasteur,Department of Vector Biology
[2] Genetics and Genomics of Insect Vectors,undefined
[3] CNRS unit URA3012,undefined
[4] Centre for Applied Entomology and Parasitology,undefined
[5] School of Life Sciences,undefined
[6] Keele University,undefined
[7] Liverpool School of Tropical Medicine,undefined
来源：
Nature Protocols | 2014年 / 9卷
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摘要：
Anopholes gambiae has proven trickier to genetically manipulate than other mosquito species. Here, Pondeville et al. provide an optimized Protocol for ΦC31-mediated trangenesis that can generate transgenic A. gambiae in 2–3 months.
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页码：1698 / 1712
页数：14
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