Development and utilization of gyrA and gyrB gene-based diagnostics for the phytoplasma classified under 16Sr I group in plants and insects

被引：0

作者：

Aundy Madhupriya

G. P. Kumar

S. M. P. Rao

机构：

[1] ICAR-Indian Agricultural Research Institute,Division of Plant Pathology

[2] Amity University,Amity Institute of Biotechnology

来源：

3 Biotech | 2019年 / 9卷

关键词：

Multilocus genes; Validation; Phytoplasma detection; Leafhopper;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

In the present study, a new set of primers of gyrA and gyrB genes of the phytoplasma genome were designed and validated for the successful detection and taxonomic classification of the previously identified phytoplasma strains of ‘Candidatus P. asteris’ (16SrI-B subgroup) associated with Catharanthus leaf yellows, sesame phyllody and the leafhopper (Hishimonus phycitis). Our results suggested the ability and sensitivity of gyrA and gyrB genes as an alternative molecular marker to identify the Ca. P. asteris strain up to subgroup level associated both with plants and insects.

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