Studying factors involved in biogenesis of Lysobacter sp. XL1 outer membrane vesicles

被引：0
|
作者：
I. V. Kudryakova
N. E. Suzina
N. G. Vinokurova
N. A. Shishkova
N. V. Vasilyeva
机构：
[1] G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms of the Russian Academy of Sciences (IBPM RAS),
[2] State Research Center for Applied Microbiology and Biotechnology,undefined
来源：
Biochemistry (Moscow) | 2017年 / 82卷
关键词：
factors of outer membrane vesicle biogenesis; bacteriolytic protease L5; cardiolipin; sp. XL1; lytic effect;
D O I：
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中图分类号：
学科分类号：
摘要：
The Gram-negative bacterium Lysobacter sp. XL1 produces outer membrane vesicles that are heterogeneous in size, density, and protein composition. One of the subpopulations is secretory vesicles for lytic protease L5 of Lysobacter sp. XL1 (Kudryakova et al. (2015) FEMS Microbiol. Lett., 362, fnv137). Protein L5 was assumed to influence biogenesis of these secretory vesicles that contain it. Using a Pseudomonas fluorescens Q2-87/B expression system, it was shown that the recombinant L5 protein may act as a factor of vesicle biogenesis. This points to a possible involvement of L5 protein in Lysobacter sp. XL1 vesicle biogenesis. Furthermore, it was established that the main phospholipid of Lysobacter sp. XL1 vesicles is cardiolipin, and vesicles are formed predominantly of outer membrane regions enriched with this phospholipid. This indicates that cardiolipin participates in biogenesis of all vesicle subpopulations in Lysobacter sp. XL1.
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页码：501 / 509
页数：8
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