Towards the development of a nuclear transformation system for Dunaliella tertiolecta

被引：0
|
作者：
Tara L. Walker
Douglas K. Becker
James L. Dale
Chris Collet
机构：
[1] Science Research Centre and CRC for Diagnostics,Cluster for Molecular Biotechnology
[2] Queensland University of Technology,undefined
来源：
Journal of Applied Phycology | 2005年 / 17卷
关键词：
transformation; electroporation; promoter;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A nuclear transformation system for the microalga Dunaliella tertiolecta was explored using electroporation. Plasmids incorporating the D. tertiolecta RbcS1 5′ and 3′ untranslated regions flanking the Streptoalloteichus hindustanus gene encoding bleomycin resistance (ble) were introduced into D. tertiolecta cells both transiently and stably. Southern hybridisation was used to examine the fate of the plasmid following electroporation and revealed that the DNA was entering the cells but was quickly degraded. Using the same procedure one stably transformed line was recovered.
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页码：363 / 368
页数：5
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