Saturation editing of genomic regions by multiplex homology-directed repair

被引：0
|
作者：
Gregory M. Findlay
Evan A. Boyle
Ronald J. Hause
Jason C. Klein
Jay Shendure
机构：
[1] University of Washington,Department of Genome Sciences
来源：
Nature | 2014年 / 513卷
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学科分类号：
摘要：
The authors perform saturation mutagenesis of genomic regions in their native endogenous chromosomal context by using CRISPR/Cas9 RNA-guided cleavage and multiplex homology-directed repair; its utility is demonstrated by measuring the effects of hundreds to thousands of genomic edits to BRCA1 and DBR1 on splicing and cellular fitness, respectively.
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页码：120 / 123
页数：3
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