Tissue culture of the deep-sea bivalve Calyptogena soyoae

被引：0
|
作者：
S. Koyama
Masuo Aizawa
机构：
[1] Frontier Research Program for Deep-sea Extremophiles,
[2] Japan Marine Science and Technology Center,undefined
[3] 2-15 Natsushima-cho,undefined
[4] Yokosuka 237-0061,undefined
[5] Japan Tel. +81-468-67-5542; Fax +81-468-66-6364 e-mail: skoyama@jamstec.go.jp,undefined
[6] Department of Biological Information,undefined
[7] Tokyo Institute of Technology,undefined
[8] Nagatsuta,undefined
[9] Yokohama,undefined
[10] Japan,undefined
来源：
Extremophiles | 2000年 / 4卷
关键词：
Key wordsCalyptogena soyoae; Deep-sea clam; Cell culture; DNA synthesis;
D O I：
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学科分类号：
摘要：
Tissue culture for the deep-sea clam Calyptogena soyoae (C. soyoae) has been examined. Mantle tissue was cultured in Dulbecco's modified Eagle medium that was prepared using artificial seawater supplemented with fetal bovine serum (FBS) and the body fluid of C. soyoae. The mantle cells were viable in culture for at least 13 days at 4°C and atmospheric pressure on a polylysine-coated dish, although no cells attached in the body fluid-free culture medium. It was found that mantle cells synthesized DNA and seemed to proliferate under atmospheric conditions.
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页码：385 / 389
页数：4
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