Development of a test strip reader for a lateral flow membrane-based immunochromatographic assay

被引：0
|
作者：
Suhyeon Kim
Je-Kyun Park
机构：
[1] LG Electronics Institute of Technology,Bio Electronics Group
[2] Korea Advanced Institute of Science and Technology,Department of BioSystems
来源：
Biotechnology and Bioprocess Engineering | 2004年 / 9卷
关键词：
test strip reader; immunoassay; immunochromatographic assay; lateral flow assay;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
A low-cost, simple strip reader system using a linear movement mechanism of CD-ROM deck has been developed to characterize a lateral flow membrane-based immunochromatographic assay. The test strip reader was assembled by a CD-ROM deck and home-made optical head especially designed for immunoassays. The optical head for detecting reflected light from the test strip surface consists of green light-emitting diode, large area silicon photodiode, and anodized aluminum mounting block providing a slit structure for cutting light from the LED. The stepping motor of the deck was operated in the full step mode, whose distance of each reading point is about 0.15 mm. The performance of the strip reader was tested by analysis of HBV (hepatitis B virus) antigen test kit. This strip reader can be useful for inexpensive, disposable, and membrane-based assays that provide visual evidence of the presence of an analyte in a liquid sample.
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页码：127 / 131
页数：4
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