Expression and characteristic of synthetic human epidermal growth factor (hEGF) in transgenic tobacco plants

被引：0
|
作者：
Jie-Ying Bai
Lin Zeng
Yuan-Lei Hu
Yan-Fang Li
Zhong-Ping Lin
Shi-Chen Shang
Yan-Sheng Shi
机构：
[1] Peking University,The National Laboratory of Protein Engineering and Plant Genetic Engineering
[2] Laboratory Animal Center of the Academy of Military Medical Science,College of Plant Sciences
[3] Jilin University,undefined
来源：
Biotechnology Letters | 2007年 / 29卷
关键词：
Biological activity; Human epidermal growth factor; Matrix attachment regions; Optimized codons; Tobacco;
D O I：
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中图分类号：
学科分类号：
摘要：
To improve the accumulation of recombinant human epidermal growth factor (hEGF) in transgenic tobacco, a highly effective vector was constructed and transformed via Agrobacterium tumefaciens. The hEGF content in transgenic tobacco was up to 0.3% of the total soluble protein. Using the Vero E6 cell expansion assay and the MTT method for cell proliferation, hEGF produced by transgenic tobacco significantly stimulated Vero E6 cell expansion and proliferation, the same as commercial hEGF products.
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