Acaricidal activity of ethanolic extract of Artemisia absinthium against Hyalomma anatolicum ticks

The aim of the study was to evaluate the in vitro efficacy of different concentrations of ethanolic extract obtained from the aerial parts of Artemisia absinthium in comparison to amitraz on adults, eggs and larvae of Hyalomma anatolicum using the adult immersion test (AIT), egg hatchability test and larval packet test (LPT), respectively. Four concentrations of the extract (2.5, 5, 10 and 20 %) with three replications for each concentration were used in all the bioassays. In AIT, the mortality rates at 2.5, 5 and 10 % were significantly different (p < 0.05) in comparison to the control group; however, at 20 %, it was similar to the positive control group. Maximum mortality of 86.7 % was recorded at 20 %. The LC50 and LC95 values were calculated as 6.51 and 55.43 %, respectively. The oviposition was reduced significantly by 36.8 and 59.1 % at concentrations of 10 and 20 %, respectively. Egg hatchability was reduced significantly at all concentrations (2.5–20 %) in comparison to the control. In LPT, the extract caused 100 % mortality of larvae at all the concentrations after 24 h. The results show that ethanolic extract obtained from the aerial parts of A. absinthium has acaricidal properties and could be useful in controlling H. anatolicum.