Using transcriptome sequencing to identify mechanisms of drug action and resistance

被引：0
|
作者：
Wacker S.A. [1 ]
Houghtaling B.R. [1 ]
Elemento O. [2 ]
Kapoor T.M. [1 ]
机构：
[1] Laboratory of Chemistry and Cell Biology, Rockefeller University, New York, NY
[2] Institute for Computational Biomedicine, Weill Cornell Medical College, New York, NY
来源：
Nature Chemical Biology | 2012年 / 8卷 / 3期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
D O I：
10.1038/nchembio.779
中图分类号：
学科分类号：
摘要：
Determining mechanisms of drug action in human cells remains a major challenge. Here we describe an approach in which multiple-drug-resistant clones are isolated and transcriptome sequencing is used to find mutations in each clone. Further analysis of mutations common to more than one clone can identify a drug's physiological target and indirect resistance mechanisms, as indicated by our proof-of-concept studies of the cytotoxic anticancer drugs BI 2536 and bortezomib.
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收藏
页码：235 / 237
页数：2
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