An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR

被引：0
|
作者：
Chen Zi
Dexin Zeng
Nan Ling
Jianjun Dai
Feng Xue
Yuan Jiang
Baoguang Li
机构：
[1] Nanjing Agricultural University,MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine
[2] Shanghai Entry-Exit Inspection and Quarantine Bureau,undefined
[3] Division of Molecular Biology,undefined
[4] Center for Food Safety and Applied Nutrition,undefined
[5] U.S. Food and Drug Administration,undefined
来源：
BMC Microbiology | / 18卷
关键词：
PMA-qPCR; Sarkosyl; Triton x-100; Accurate detection; Viability; False positive; Milk; Methicillin-resistant ;
D O I：
暂无
中图分类号：
学科分类号：
摘要：
引用
收藏
相关论文
共 50 条
  • [1] An improved assay for rapid detection of viable Staphylococcus aureus cells by incorporating surfactant and PMA treatments in qPCR
    Zi, Chen
    Zeng, Dexin
    Ling, Nan
    Dai, Jianjun
    Xue, Feng
    Jiang, Yuan
    Li, Baoguang
    BMC MICROBIOLOGY, 2018, 18
  • [2] Development of PMA-LAMP for Rapid Detection of Staphylococcus aureus in Viable but Nonculturable State
    Zhong, Qing-Ping
    Chen, Ying-Qiao
    Wang, Li
    Fang, Xiang
    Liao, Zhen-Lin
    JOINT 2016 INTERNATIONAL CONFERENCE ON SOCIAL SCIENCE AND ENVIRONMENTAL SCIENCE (SSES 2016) AND INTERNATIONAL CONFERENCE ON FOOD SCIENCE AND ENGINEERING (ICFSE 2016), 2016, : 451 - 456
  • [3] Rapid and quantitative detection of viable emetic Bacillus cereus by PMA-qPCR assay in milk
    Zhou, Ping
    Xie, Guoyang
    Liang, Taobo
    Yu, Bei
    Aguilar, Zoraida
    Xu, Hengyi
    MOLECULAR AND CELLULAR PROBES, 2019, 47
  • [4] Optimization of PMA-qPCR for Staphylococcus aureus and determination of viable bacteria in indoor air
    Chang, C. -W.
    Lin, M. -H.
    INDOOR AIR, 2018, 28 (01) : 64 - 72
  • [5] Practical application of PMA-qPCR assay for determination of viable cells of inter-species biofilm of Candida albicans-Staphylococcus aureus
    Kendra, Samuel
    Varga, Jarmila Czucz
    Gaalova-Radochova, Barbora
    Bujdakova, Helena
    BIOLOGY METHODS & PROTOCOLS, 2024, 9 (01):
  • [6] Rapid Detection of Viable Penicillium expansum in Apple by PMA-qPCR
    Yang J.
    Ding Y.
    Yu L.
    Fan Y.
    Liu F.
    Zhao X.
    Jiao Z.
    Wang C.
    Science and Technology of Food Industry, 2023, 44 (16) : 331 - 338
  • [7] Multiplex PMA-qPCR Assay with Internal Amplification Control for Simultaneous Detection of Viable Legionella pneumophila, Salmonella typhimurium, and Staphylococcus aureus in Environmental Waters
    Li, Haiyan
    Xin, Hongyi
    Li, Sam Fong Yau
    ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2015, 49 (24) : 14249 - 14256
  • [8] Rapid and visual detection of viable Staphylococcus aureus in pork and pork products by PMA and saltatory rolling circle amplification
    Wei Guo
    Qian Yang
    Yunzhe Zhang
    Xin Lu
    Chenchen Wu
    Jianxin Tan
    Wei Zhang
    European Food Research and Technology, 2022, 248 : 1625 - 1634
  • [9] Rapid and visual detection of viable Staphylococcus aureus in pork and pork products by PMA and saltatory rolling circle amplification
    Guo, Wei
    Yang, Qian
    Zhang, Yunzhe
    Lu, Xin
    Wu, Chenchen
    Tan, Jianxin
    Zhang, Wei
    EUROPEAN FOOD RESEARCH AND TECHNOLOGY, 2022, 248 (06) : 1625 - 1634
  • [10] Rapid and Simple Detection of Viable Foodborne Pathogen Staphylococcus aureus
    Liu, Caiyan
    Shi, Chao
    Li, Mengzhe
    Wang, Mengyuan
    Ma, Cuiping
    Wang, Zonghua
    FRONTIERS IN CHEMISTRY, 2019, 7
12345