Gene Cloning and Characterization of a Poly(l-Lactic Acid) Depolymerase from Pseudomonas sp. Strain DS04-T

被引：0
|
作者：
Zhan-Yong Wang
Fan Li
Zi-Qi Guo
Yan Wang
Shan Chen
机构：
[1] Northeast Normal University,School of Life Sciences
[2] Liaoning Shihua University,School of Environmental and Biological Engineering
来源：
Journal of Polymers and the Environment | 2011年 / 19卷
关键词：
Poly(; -lactic acid); Depolymerase; sp. strain DS04-T; Recombinant;
D O I：
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学科分类号：
摘要：
A gene encoding a poly(l-lactic acid) (PLA) depolymerase from Pseudomonas sp. strain DS04-T was cloned and overexpressed in Escherichia coli. The recombinant PLA depolymerase with a molecular weight of 19.2 kDa was purified to homogeneity. The optimum pH and temperature of the PLA depolymerase are 8.5 and 60 °C, respectively. K+, Ca2+ and Ni2+ enhance the enzyme activity, while Na+, Zn2+, Mg2+, Cu2+, Fe2+, Mn2+ and Co2+ inhibit it. The inhibition of different chemicals on the PLA depolymerase activity were examined, in which EDTA was found to have a significantly inhibitory effect. The main degradation product of the depolymerase is identified as lactic acid monomer by mass spectrometric analysis. Physicochemical properties, substrate specificity and sequence analysis indicated that PME is a new type of PLA depolymerase.
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页码：827 / 833
页数：6
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