Purification and properties of an endo-inulinase from an Arthrobacter sp.

被引:0
|
作者
Su-II Kang
Yung-Jin Chang
Sun-Jin Oh
Su-II Kim
机构
[1] Seoul National University,Department of Agricultural Chemistry and Research Center for New Bio
来源
Biotechnology Letters | 1998年 / 20卷
关键词
endo-inulinase; inulo-oligosaccharides; Arthrobacter sp.;
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摘要
Extracellular endo-inulinase of Arthrobacter sp. S37 was purified 63-fold, giving a single band on PAGE with activity staining. The Mr was estimated as 75 kDa by SDS-PAGE. The first 31 amino acids of the N-terminal sequence was determined. The endo-inulinase hydrolyzed inulin mainly into inulo-triose (F3), inulo-tetraose (F4) and inulo-pentaose (F5) optimally at pH 7.5 and 50°C. © Rapid Science Ltd. 1998
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页码:983 / 986
页数:3
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