Alumina nanoparticle-assisted enzyme refolding: A versatile methodology for proteins renaturation

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作者：
Katerina V. Volodina
David Avnir
Vladimir V. Vinogradov
机构：
[1] ITMO University,
[2] Laboratory of Solution Chemistry of Advanced Materials and Technologies,undefined
[3] Institute of Chemistry and the Center for Nanoscience and Nanotechnology,undefined
[4] the Hebrew University of Jerusalem,undefined
来源：
Scientific Reports | / 7卷
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摘要：
We present a high-yield method for the renaturation of negatively charged enzymes. The approach is based on the use of alumina nanoparticles, which after electrostatic interaction with denatured protein molecules, prevent their aggregation and make the process of refolding controllable. The method, demonstrated by the renaturation of several enzymes, is efficient, rapid, employs a minimal amount of reagents and even can be applied to renature mixture of the denatured enzymes.
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