Isolation, cloning, sequencing of brain type aromatase and its expression in male and female Wrasse, Pseudolabrus sieboldi

被引:0
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作者
J. K. Sundaray
K. Ohta
A. Yamaguchi
T. Kitano
M. Matsuyama
机构
[1] Central Institute of Brackish Water Aquaculture,Kakdwip Research Center
[2] National Institute of Basic Biology,Laboratory of Marine Biology, Faculty of Agriculture
[3] Kyushu University,Graduate School of Science and Technology
[4] Kumamoto University,undefined
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关键词
Brain; Aromatase; Wrasse; Diurnal rhythm;
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摘要
Pseudolabrus sieboldi, wrasse being a diurnal spawner provides a good opportunity to study the endocrine mechanism of estrogen formation in brain and gonads. Moreover, an extremely large amount of E2 was produced in serum and testis of wrasse. It is assumed that the presence of E2 may play a major role in diurnal gametogenesis in male fish. In this study brain type aromatase have been isolated, cloned and sequenced from the brain of wrasse. Further, the expression pattern of brain type aromatase in gonads and adult tissue of male and female fish have been analyzed. In addition, the diurnal expression pattern of brain type aromatase in both male and female fish brain during spawning season have been analyzed.
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页码:137 / 141
页数:4
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